Aspergillus strains belonging to the Aspergillus niger aggregate, either isolated from Italian grapes or received from public collections, were analysed in order to discriminate between the ochratoxin A (OTA) producing and the non-producing strains by means of the analysis of Internal Transcribed Spacers (ITS), Intergenic Spacers (IGS) and of a β-tubulin gene portion. A. niger and Aspergillus awamori were identified observing the macro- and microscopic features of the colonies and the strains ochratoxigenicity was evaluated through Thin Layer Chromatography and/or High Performance Liquid Chromatography. PCR amplification of ITS, IGS and β-tubulin gene portion produced 600, 440 and 550 bp amplicons, respectively, in all the analysed strains. The digestion of the IGS amplicon with Hinf I and of the other two amplicons with Rsa I, revealed one main profile type (“T–C–C”) associated with the A. niger aggregate strains which lack the ability to produce OTA.
|Data di pubblicazione:||2006|
|Titolo:||Digestion of DNA regions to discriminate ochratoxigenic and non-ochratoxigenic strains in the Aspergillus niger aggregate|
|Autori:||ZANZOTTO, A; BURRUANO, S; MARCIANO, P|
|Tipologia:||Articolo su rivista|
|Citazione:||ZANZOTTO, A., BURRUANO, S., & MARCIANO, P. (2006). Digestion of DNA regions to discriminate ochratoxigenic and non-ochratoxigenic strains in the Aspergillus niger aggregate. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 110, 155-159.|
|Digital Object Identifier (DOI):||0.1016/j.ijfoodmicro.2006.04.003|
|Appare nelle tipologie:||01 - Articolo su rivista|