Callus induction, somatic embryogenesis and plant regeneration were obtained in Citrus limon (L.) Burm. (cv. Femminello) from cultures of pistil transverse thin cell layer explants [(t)TCLs]. Explants were cultured on two different media, based on Murashige and Skoog salts and vitamins, supplemented with 500 mg l−1 malt extract (MSI), or 500 mg l−1 malt extract and 13.3 μM 6-benzylaminopurine (MSII). Sucrose (146 mM) was used as carbon source. Somatic embryos appeared 3 months after culture initiation from stigma and style (t)TCLs; they were observed at the surface of the (t)TCL-derived callus. Although ovary (t)TCLs showed the highest callus formation, they never differentiated somatic embryos. Percentages of embryo formation from (t)TCLs incubated on MSI (13% and 2% for stigma and style, respectively) were lower than those from (t)TCLs incubated on MSII (36% and 7% for stigma and style, respectively). The embryogenic response of stigma (t)TCLs was usually higher than that of style (t)TCLs. After about 3 months, somatic embryos developed into plantlets at high frequencies (57% and 62% for stigma- and style-derived somatic embryos, respectively).
|Data di pubblicazione:||2008|
|Titolo:||Somatic embryogenesis and plant regeneration from pistil transverse thin cell layers of lemon (Citrus limon).|
|Autori:||Sajeva, M.; Carra, A.; DE PASQUALE, F.; Carimi, F.|
|Tipologia:||Articolo su rivista|
|Citazione:||Sajeva, M., Carra, A., DE PASQUALE, F., & Carimi, F. (2008). Somatic embryogenesis and plant regeneration from pistil transverse thin cell layers of lemon (Citrus limon). PLANT BIOSYSTEMS, 142, 199-203.|
|Digital Object Identifier (DOI):||10.1080/11263500802150258|
|Appare nelle tipologie:||01 - Articolo su rivista|