(1) Background: A new family of nanosystems able to discern between normal and tumor cells and to release a therapeutic agent in controlled way were synthetized by e-beam irradiation. This technique permits to obtain biocompatible, sterile, carboxyl-functionalized polyvinylpyrrolidone (PVP-co-acrylic acid) nanogels (NGs); (2) Methods: Here, we performed a targeting strategy based on the recognition of over-expressed proteins on tumor cells, like the folate receptor. The selective targeting was demonstrated by co-culture studies and flow cytometry analysis, using folate conjugated NGs. Moreover, nanoparticles were conjugated to a chemotherapeutic drug or to a pro-apoptotic siRNA through a glutathione sensitive spacer, in order to obtain a controlled release mechanism, specific for cancer cells. The drug efficiency was tested on tumor and healthy cells by flow cytometric analysis, confocal and epifluorescence microscopy and cytotoxicity assay; the siRNA effect was investigated by RNAi experiment; (3) Results: The data obtained showed that the use of NGs permits a faster cargo release in cancer cells, in response to high cytosolic glutathione level, also improving their efficacy; (4) Conclusion: The possibility of releasing biological molecules in a controlled way and to recognize a specific tumor target allows overcoming the typical limits of the classic cancer therapy.

Adamo, G., Grimaldi, N., Campora, S., Bulone, D., Bondi, M.L., Al-Sheikhly, M., et al. (2016). Multi-functional nanogels for tumor targeting and redox-sensitive drug and siRNA delivery. MOLECULES, 21(11), 1594 [10.3390/molecules21111594].

Multi-functional nanogels for tumor targeting and redox-sensitive drug and siRNA delivery

ADAMO, Giorgia;CAMPORA, Simona;SABATINO, Maria Antonietta;DISPENZA, Clelia;GHERSI, Giulio
2016-01-01

Abstract

(1) Background: A new family of nanosystems able to discern between normal and tumor cells and to release a therapeutic agent in controlled way were synthetized by e-beam irradiation. This technique permits to obtain biocompatible, sterile, carboxyl-functionalized polyvinylpyrrolidone (PVP-co-acrylic acid) nanogels (NGs); (2) Methods: Here, we performed a targeting strategy based on the recognition of over-expressed proteins on tumor cells, like the folate receptor. The selective targeting was demonstrated by co-culture studies and flow cytometry analysis, using folate conjugated NGs. Moreover, nanoparticles were conjugated to a chemotherapeutic drug or to a pro-apoptotic siRNA through a glutathione sensitive spacer, in order to obtain a controlled release mechanism, specific for cancer cells. The drug efficiency was tested on tumor and healthy cells by flow cytometric analysis, confocal and epifluorescence microscopy and cytotoxicity assay; the siRNA effect was investigated by RNAi experiment; (3) Results: The data obtained showed that the use of NGs permits a faster cargo release in cancer cells, in response to high cytosolic glutathione level, also improving their efficacy; (4) Conclusion: The possibility of releasing biological molecules in a controlled way and to recognize a specific tumor target allows overcoming the typical limits of the classic cancer therapy.
2016
Settore CHIM/07 - Fondamenti Chimici Delle Tecnologie
Adamo, G., Grimaldi, N., Campora, S., Bulone, D., Bondi, M.L., Al-Sheikhly, M., et al. (2016). Multi-functional nanogels for tumor targeting and redox-sensitive drug and siRNA delivery. MOLECULES, 21(11), 1594 [10.3390/molecules21111594].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/215973
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