Background Huanglongbing (HLB) disease is still the greatest threat to citriculture worldwide. Although there is not any resistance source in the Citrus germplasm, a certain level of moderated tolerance is present. A large-scale analysis of proteomic responses of Citrus may help: 1) clarifying physiological and molecular effects of disease progression, 2) validating previous data at transcriptomic level, and 3) identifying biomarkers for development of early diagnostics, short-term therapeutics and long-term genetic resistance. Results In this work we have conducted a proteomic analysis of mature leaves of two Citrus genotypes with well-known differing tolerances to HLB: Navel orange (highly susceptible) and Volkameriana (moderately tolerant). Pathway enrichment analysis showed that amino acid degradation processes occurred to a larger degree in the Navel orange. No clear differences between the two genotypes were observed for primary metabolic pathways. The most important finding was that four glutathione-S-transferases were upregulated in Volkameriana and not in Navel orange. These proteins are involved in radical ion detoxification. Conclusions Upregulation of proteins involved in radical ion detoxification should be considered as an important mechanism of increased tolerance to HLB.

Martinelli, F., Reagan, R.L., Dolan, D., Fileccia, V., Dandekar, A. (2016). Proteomic analysis highlights the role of detoxification pathways in increased tolerance to Huanglongbing disease. BMC PLANT BIOLOGY(16) [10.1186/s12870-016-0858-5].

Proteomic analysis highlights the role of detoxification pathways in increased tolerance to Huanglongbing disease

MARTINELLI, Federico;Fileccia, Veronica;
2016-01-01

Abstract

Background Huanglongbing (HLB) disease is still the greatest threat to citriculture worldwide. Although there is not any resistance source in the Citrus germplasm, a certain level of moderated tolerance is present. A large-scale analysis of proteomic responses of Citrus may help: 1) clarifying physiological and molecular effects of disease progression, 2) validating previous data at transcriptomic level, and 3) identifying biomarkers for development of early diagnostics, short-term therapeutics and long-term genetic resistance. Results In this work we have conducted a proteomic analysis of mature leaves of two Citrus genotypes with well-known differing tolerances to HLB: Navel orange (highly susceptible) and Volkameriana (moderately tolerant). Pathway enrichment analysis showed that amino acid degradation processes occurred to a larger degree in the Navel orange. No clear differences between the two genotypes were observed for primary metabolic pathways. The most important finding was that four glutathione-S-transferases were upregulated in Volkameriana and not in Navel orange. These proteins are involved in radical ion detoxification. Conclusions Upregulation of proteins involved in radical ion detoxification should be considered as an important mechanism of increased tolerance to HLB.
2016
Settore AGR/07 - Genetica Agraria
Martinelli, F., Reagan, R.L., Dolan, D., Fileccia, V., Dandekar, A. (2016). Proteomic analysis highlights the role of detoxification pathways in increased tolerance to Huanglongbing disease. BMC PLANT BIOLOGY(16) [10.1186/s12870-016-0858-5].
File in questo prodotto:
File Dimensione Formato  
Martinelli et al., 2016 BMC.pdf

Solo gestori archvio

Dimensione 3.03 MB
Formato Adobe PDF
3.03 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/202490
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 43
  • ???jsp.display-item.citation.isi??? 33
social impact