tThe diversification of cellular functions is one of the major characteristics of multicellular organ-isms which allow cells to modulate their gene expression, leading to the formation of transcripts andproteins with different functions and concentrations in response to different stimuli. CAP genes rep-resent a widespread family of proteins belonging to the cysteine-rich secretory protein, antigen 5 andpathogenesis-related 1 superfamily which, it has been proposed, play key roles in the infection processand the modulation of immune responses in host animals. The ascidian Ciona intestinalis represents agroup of proto-chordates with an exclusively innate immune system that has been widely studied inthe field of comparative and developmental immunology. Using this biological system, we describe theidentification of a novel APA mechanism by which an intronic polyadenylation signal is activated by LPSinjection, leading to the formation of a shorter CAP mRNA capable of expressing the first CAP exon plus19 amino acid residues whose sequence is contained within the first intron of the annotated gene. Fur-thermore, such an APA event causes the expression of a translational controlling cis-acting GAIT elementwhich is not present in the previously isolated CAP isoform and identified in the 3-UTR of other immune-related genes, suggesting an intriguing scenario in which both transcriptional and post-transcriptionalcontrol mechanisms are involved in the activation of the CAP gene during inflammatory response in C.intestinalis.

Vizzini, A., Bonura, A., Longo, V., Sanfratello, M.A., Parrinello, D., Cammarata, M., et al. (2016). LPS injection reprograms the expression and the 3'UTR of a CAP gene by alternative polyadenylation and the formation of a GAIT element in Ciona intestinalis. MOLECULAR IMMUNOLOGY, 77, 174-183 [10.1016/j.molimm.2016.08.004].

LPS injection reprograms the expression and the 3'UTR of a CAP gene by alternative polyadenylation and the formation of a GAIT element in Ciona intestinalis

VIZZINI, Aiti;PARRINELLO, Daniela;CAMMARATA, Matteo;
2016-01-01

Abstract

tThe diversification of cellular functions is one of the major characteristics of multicellular organ-isms which allow cells to modulate their gene expression, leading to the formation of transcripts andproteins with different functions and concentrations in response to different stimuli. CAP genes rep-resent a widespread family of proteins belonging to the cysteine-rich secretory protein, antigen 5 andpathogenesis-related 1 superfamily which, it has been proposed, play key roles in the infection processand the modulation of immune responses in host animals. The ascidian Ciona intestinalis represents agroup of proto-chordates with an exclusively innate immune system that has been widely studied inthe field of comparative and developmental immunology. Using this biological system, we describe theidentification of a novel APA mechanism by which an intronic polyadenylation signal is activated by LPSinjection, leading to the formation of a shorter CAP mRNA capable of expressing the first CAP exon plus19 amino acid residues whose sequence is contained within the first intron of the annotated gene. Fur-thermore, such an APA event causes the expression of a translational controlling cis-acting GAIT elementwhich is not present in the previously isolated CAP isoform and identified in the 3-UTR of other immune-related genes, suggesting an intriguing scenario in which both transcriptional and post-transcriptionalcontrol mechanisms are involved in the activation of the CAP gene during inflammatory response in C.intestinalis.
2016
Settore BIO/05 - Zoologia
Vizzini, A., Bonura, A., Longo, V., Sanfratello, M.A., Parrinello, D., Cammarata, M., et al. (2016). LPS injection reprograms the expression and the 3'UTR of a CAP gene by alternative polyadenylation and the formation of a GAIT element in Ciona intestinalis. MOLECULAR IMMUNOLOGY, 77, 174-183 [10.1016/j.molimm.2016.08.004].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/196553
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