We have previously shown that PTHrP(38-94)-amide restrains growth and invasion "in vitro", causes striking toxicity and accelerates death of some breast cancer cell lines, the most responsive being MDA-MB231 whose tumorigenesis was also attenuated "in vivo". PTHrP(38-94)-amide contains the domain implicated in the nuclear import of PTHrP. Although the nucleus was identified as a destination for mid-region PTHrP, evidence for direct DNA-binding capability is lacking to date. Here, we examined the localization of PTHrP(38-94)-amide within MDA-MB231 cells and within metaphase spread preparations and characterized its DNA-binding properties, employing a combination of immunocytochemical, cytogenetic, "whole genome"/conventional PCR, EMSA and DNase footprinting techniques. The results obtained: (i) show that PTHrP(38-94)-amide gains access to the nuclear compartment of MDA-MB231 cell; (ii) demonstrate that PTHrP(38-94)-amide is a DNA-binding peptide; and, (iii) represent the first data to date on the potential molecular targets in both cellular chromatin and isolated oligonucleotides "in vitro".
|Data di pubblicazione:||2006|
|Titolo:||Mid-region parathyroid hormone-related protein (PTHrP) binds chromatin of MDA-MB231 breast cancer cells and isolated oligonucleotides "in vitro"|
|Autori:||SIRCHIA, R; PRIULLA, M; SCIANDRELLO, G; CARADONNA, F; BARBATA, G; LUPARELLO, C|
|Tipologia:||Articolo su rivista|
|Citazione:||SIRCHIA, R., PRIULLA, M., SCIANDRELLO, G., CARADONNA, F., BARBATA, G., & LUPARELLO, C. (2006). Mid-region parathyroid hormone-related protein (PTHrP) binds chromatin of MDA-MB231 breast cancer cells and isolated oligonucleotides "in vitro". BREAST CANCER RESEARCH AND TREATMENT, 2006, 00-00.|
|Digital Object Identifier (DOI):||10.1007|
|Appare nelle tipologie:||01 - Articolo su rivista|