Interleukin (IL)-22 receptor 1 is over-expressed in primary Sjogren's syndrome and Sjögren-associated non-Hodgkin lymphomas and is regulated by IL-18.

Ciccia, F; Guggino, G; Rizzo, A; Bombardieri, M; Raimondo, S; Carubbi F, CA; Sireci, G; Dieli, F; Campisi, G; Giacomelli, R; Cipriani, P; De Leo, G; Alessandro, R; Triolo, G

doi:10.1111/cei.12643

The aim of this study was to elucidate more clearly the role of interleukin (IL)-18 in modulating the IL-22 pathway in primary Sjögren's syndrome (pSS) patients and in pSS-associated lymphomas. Minor salivary glands (MSGs) from patients with pSS and non-specific chronic sialoadenitis (nSCS), parotid glands biopsies from non-Hodgkin lymphomas (NHL) developed in pSS patients, were evaluated for IL-18, IL-22, IL-22 receptor 1 (IL-22R1), IL-22 binding protein (IL-22BP) and signal transducer and activator of transcription-3 (STAT-3) expression. MSGs IL-22R1-expressing cells were characterized by confocal microscopy and flow cytometry in pSS, nSCS and healthy controls . The effect of recombinant IL-18 and IL-22 on peripheral blood mononuclear cells (PBMCs) from pSS and nSCS was studied by flow cytometry and reverse transcription-polymerase chain reaction (RT-PCR). MSGs of pSS and NHL were characterized by an imbalance between IL-22 and IL-22BP protein expression, with IL-18 and IL-22BP being expressed in a mutually exclusive manner and IL-18 and IL-22R1 being correlated directly. Aberrant expression of IL-22R1, induced by IL-18, was observed only among tissue and circulating myeloid cells of pSS patients and macrophages of NHL tissues of pSS patients, but not nSCS. IL-22R1 expression on PBMC of pSS was functional, as its stimulation with recombinant IL-22 significantly up-regulated the expression of STAT-3, IL-17 and IL-22. An IL-18-dependent aberrant expression of IL-22R1 on cells of haematopoietic origin seems to be a specific immunological signature of patients with pSS and pSS-associated lymphomas.

Ciccia F, Guggino G, Rizzo A, Bombardieri M, Raimondo S, Carubbi F, C.A., et al. (2015). Interleukin (IL)-22 receptor 1 is over-expressed in primary Sjogren's syndrome and Sjögren-associated non-Hodgkin lymphomas and is regulated by IL-18. CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 181(2), 219-229 [10.1111/cei.12643].

Data di pubblicazione:	2015
Titolo:	Interleukin (IL)-22 receptor 1 is over-expressed in primary Sjogren's syndrome and Sjögren-associated non-Hodgkin lymphomas and is regulated by IL-18.
Autori:	CICCIA, Francesco GUGGINO, Giuliana Rizzo A Bombardieri M RAIMONDO, Stefania Carubbi F, Cannizzaro A SIRECI, Guido DIELI, Francesco CAMPISI, Giuseppina Giacomelli R Cipriani P DE LEO, Giacomo ALESSANDRO, Riccardo TRIOLO, Giovanni (Corresponding) mostra contributor esterni nascondi contributor esterni
Citazione:	Ciccia F, Guggino G, Rizzo A, Bombardieri M, Raimondo S, Carubbi F, C.A., et al. (2015). Interleukin (IL)-22 receptor 1 is over-expressed in primary Sjogren's syndrome and Sjögren-associated non-Hodgkin lymphomas and is regulated by IL-18. CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 181(2), 219-229 [10.1111/cei.12643].
Rivista:	CLINICAL AND EXPERIMENTAL IMMUNOLOGY
Digital Object Identifier (DOI):	http://dx.doi.org/10.1111/cei.12643
Abstract:	The aim of this study was to elucidate more clearly the role of interleukin (IL)-18 in modulating the IL-22 pathway in primary Sjögren's syndrome (pSS) patients and in pSS-associated lymphomas. Minor salivary glands (MSGs) from patients with pSS and non-specific chronic sialoadenitis (nSCS), parotid glands biopsies from non-Hodgkin lymphomas (NHL) developed in pSS patients, were evaluated for IL-18, IL-22, IL-22 receptor 1 (IL-22R1), IL-22 binding protein (IL-22BP) and signal transducer and activator of transcription-3 (STAT-3) expression. MSGs IL-22R1-expressing cells were characterized by confocal microscopy and flow cytometry in pSS, nSCS and healthy controls . The effect of recombinant IL-18 and IL-22 on peripheral blood mononuclear cells (PBMCs) from pSS and nSCS was studied by flow cytometry and reverse transcription-polymerase chain reaction (RT-PCR). MSGs of pSS and NHL were characterized by an imbalance between IL-22 and IL-22BP protein expression, with IL-18 and IL-22BP being expressed in a mutually exclusive manner and IL-18 and IL-22R1 being correlated directly. Aberrant expression of IL-22R1, induced by IL-18, was observed only among tissue and circulating myeloid cells of pSS patients and macrophages of NHL tissues of pSS patients, but not nSCS. IL-22R1 expression on PBMC of pSS was functional, as its stimulation with recombinant IL-22 significantly up-regulated the expression of STAT-3, IL-17 and IL-22. An IL-18-dependent aberrant expression of IL-22R1 on cells of haematopoietic origin seems to be a specific immunological signature of patients with pSS and pSS-associated lymphomas.
URL dell'editore:	https://academic.oup.com/cei/article/181/2/219/6422175
URL:	https://onlinelibrary.wiley.com/doi/full/10.1111/cei.12643
Settore Scientifico Disciplinare:	Settore MED/16 - Reumatologia
Appare nelle tipologie:	1.01 Articolo in rivista

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http://hdl.handle.net/10447/190164

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