Cadmium is a heavy metal toxic for living organisms even at low concentrations. It does not have any biological role, and since it is a permanent metal ion, it is accumulated by many organisms. In the present paper we have studied the apoptotic effects of continuous exposure to subacute/sublethal cadmium concentrations on a model system: Paracentrotus lividus embryos. We demonstrated, by atomic absorption spectrometry, that the intracellular amount of metal increased during exposure time. We found, using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay, that long treatments with cadmium triggered a severe DNA fragmentation. We demonstrated, by immunocytochemistry on whole-mount embryos, that treatment with cadmium causes activation of caspase-3 and cleavage of death substrates alpha-fodrin and lamin A. Incubating the embryos since fertilization with Z-DEVD FMK, a caspase-3 inhibitor, we found, by immunocytochemistry, that cleavage by caspase-3 and cleavage of death substrates were inactivated.
|Data di pubblicazione:||2007|
|Titolo:||Cadmium induces apoptotic response in sea urchin embryos.|
|Autori:||AGNELLO, M; FILOSTO, S; SCUDIERO, R; RINALDI, AM; ROCCHERI, MC|
|Tipologia:||Articolo su rivista|
|Citazione:||AGNELLO, M., FILOSTO, S., SCUDIERO, R., RINALDI, A.M., & ROCCHERI, M.C. (2007). Cadmium induces apoptotic response in sea urchin embryos. CELL STRESS & CHAPERONES, 12, 44-50.|
|Digital Object Identifier (DOI):||10.1379/CSC-229R.1|
|Appare nelle tipologie:||01 - Articolo su rivista|