Time-resolved structural information is key to understand the mechanism of biological processes, such as catalysis and signalling. Recent developments in X-ray sources as well as data collection and analysis methods are making routine time-resolved X-ray crystallography and solution scattering experiments a real possibility for structural biologists. Here we review the information that can be obtained from these techniques and discuss the considerations that must be taken into account when designing a time-resolved experiment.
Levantino, M., Yorke, B.A., Monteiro, D., Cammarata, M., Pearson, A.R. (2015). Using synchrotrons and XFELs for time-resolved X-ray crystallography and solution scattering experiments on biomolecules. CURRENT OPINION IN STRUCTURAL BIOLOGY, 35, 41-48 [10.1016/j.sbi.2015.07.017].
Using synchrotrons and XFELs for time-resolved X-ray crystallography and solution scattering experiments on biomolecules
LEVANTINO, Matteo;
2015-01-01
Abstract
Time-resolved structural information is key to understand the mechanism of biological processes, such as catalysis and signalling. Recent developments in X-ray sources as well as data collection and analysis methods are making routine time-resolved X-ray crystallography and solution scattering experiments a real possibility for structural biologists. Here we review the information that can be obtained from these techniques and discuss the considerations that must be taken into account when designing a time-resolved experiment.
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