Expression of Ta2 gene, during sea urchin Paracentrotus lividus development, is spatially and temporally regulated. In order to characterize this gene, we isolated the relevant genomic sequences and scanned the isolated 50-flanking region in searching for cisregulatory elements required for proper expression. Gel mobility shift and footprinting assays, as well as reporter gene (CAT and beta-gal) expression assays, were used to address cis-regulatory elements involved in regulation. Here we report that an upstream 50-flanking fragment of PlTa2 gene drives temporal expression of reporter genes congruent with that of endogenous Ta2 gene. The fragment contains cis-elements able to bind nuclear proteins from the gastrula stage (at which the Ta2 gene is expressed) whose sequences could be consistent with the consensus sequences for transcription factors present in data bank.
|Data di pubblicazione:||2004|
|Titolo:||Sea urchin neural alpha 2 tubulin gene: isolation and promoter analysis|
|Autori:||COSTA S; RAGUSA MA; DRAGO G; CASANO C; ALAIMO G; GUIDA N; GIANGUZZA F|
|Tipologia:||Articolo su rivista|
|Citazione:||COSTA S, RAGUSA MA, DRAGO G, CASANO C, ALAIMO G, GUIDA N, et al. (2004). Sea urchin neural alpha 2 tubulin gene: isolation and promoter analysis. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 316(2), 446-453.|
|Digital Object Identifier (DOI):||10.1016/j.bbrc.2004.02.070|
|Appare nelle tipologie:||01 - Articolo su rivista|