Immunohistochemical and in situ hybridization assays were performed to answer the question whether the endostyle, that is the initial gastro-intestinal trait of Ciona intestinalis pharynx, is involved in galectin (CiLgals-a and CiLgals-b) production during the pharynx inflammatory response to LPS inoculation. Specific anti-CiLgal-a and anti-CiLgals-b antibodies, and oligonucleotide probes, that mark inflammatory hemocytes inside the pharynx vessels and vessel epithelium as shown by a previous paper, were assayed on endostyle histological sections. For the first time, we show that galectins are produced by endostyle zones, and both CiLgals-a and eb genes are upregulated by LPS. CiLgals-a and CiLgals-b are constitutively expressed in the endostyle zone 2 and 3, respectively, both genes are upregulated by LPS in the zone 2, and CiLgals-b in the zone 3 and 4. The antibody-reacting material contained in intracellular and extracellular large vesicles suggest an unexpected vesicle-dependent transporting mechanism of galectins not provided with signal peptide. Differential expression and gene upregulation in not-treated and LPStreated specimens, support the role of endostyle galectins both in filter feeding and defense responses.
|Data di pubblicazione:||2015|
|Titolo:||Ciona intestinalis galectin (CiLgals-a and CiLgals-b) genes are differentially expressed in endostyle zones and challenged by LPS|
CAMMARATA, Matteo (Corresponding)
|Citazione:||Parrinello D, Sanfratello M A, Vizzini A, Parrinello N., & Cammarata M (2015). Ciona intestinalis galectin (CiLgals-a and CiLgals-b) genes are differentially expressed in endostyle zones and challenged by LPS. FISH AND SHELLFISH IMMUNOLOGY, 42(1), 171-176.|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.fsi.2014.10.026|
|Settore Scientifico Disciplinare:||Settore BIO/05 - Zoologia|
|Appare nelle tipologie:||1.01 Articolo in rivista|