A series of nanoparticles is prepared via layer-by-layer assembly of oppositely charged, synthetic biocompatible polyamidoamine polymers as potential carriers. Particle size, surface charge and internal chain mobility are quantified as a function of the polymer type and number of layers. The effect of addition of surfactant is examined to simulate the effects of nanoparticle dissolution. The cyctotoxicity of these particles (in epithelia and murine cell lines) are orders of magnitude lower than polyethyleneimine controls. Stable nanoparticles may be prepared from mixtures of strongly, oppositely charged polymers, but less successfully from weakly charged polymers, and, given their acceptable toxicity characteristics, such modularly designed constructs show promise for drug and gene delivery. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Griffiths, P., Mauro, N., Murphy, D., Carter, E., Richardson, S., Dyer, P., et al. (2013). Self-assembled PAA-based nanoparticles as potential gene and protein delivery systems. MACROMOLECULAR BIOSCIENCE, 13(5), 641-649 [10.1002/mabi.201200462].
Self-assembled PAA-based nanoparticles as potential gene and protein delivery systems
Mauro, Nicolò;
2013-01-01
Abstract
A series of nanoparticles is prepared via layer-by-layer assembly of oppositely charged, synthetic biocompatible polyamidoamine polymers as potential carriers. Particle size, surface charge and internal chain mobility are quantified as a function of the polymer type and number of layers. The effect of addition of surfactant is examined to simulate the effects of nanoparticle dissolution. The cyctotoxicity of these particles (in epithelia and murine cell lines) are orders of magnitude lower than polyethyleneimine controls. Stable nanoparticles may be prepared from mixtures of strongly, oppositely charged polymers, but less successfully from weakly charged polymers, and, given their acceptable toxicity characteristics, such modularly designed constructs show promise for drug and gene delivery. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.File | Dimensione | Formato | |
---|---|---|---|
Griffiths_et_al-2013-Macromolecular_Bioscience.pdf
Solo gestori archvio
Dimensione
369.7 kB
Formato
Adobe PDF
|
369.7 kB | Adobe PDF | Visualizza/Apri Richiedi una copia |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.