Abstract Here we have identified a serum fraction containing ,8-kDa molecules with an unexpected capacity to greatly enhance particle uptake in trout head kidney leukocytes (HKLs). This ,8-kDa particle-uptake enhancing fraction (PUEF-8) was purified from complement-activated serum by gel filtration chromatography. Mass spectrometric analysis and reactivity of antitrout C3-1 and C4 antibodies, indicated the presence of C3a, C4a and C5a molecules in PUEF-8. Using a newly developed flow cytometric assay that measures the capacity of cells to ingest fluorescent beads, we showed that PUEF-8 induced a striking enhancement (344 ^ 50% higher than the PBS control value) in the number of HKLs ingesting three or more beads. In contrast, the effect of PUEF-8 on peripheral blood leukocytes (PBLs) was almost negligible. Interestingly, PUEF-8 acted as a strong chemoattractant for both HKLs and PBLs. These findings suggest a novel role for the anaphylatoxins generated during complement activation in teleost fish.
|Data di pubblicazione:||2004|
|Titolo:||Anaphylatoxin-like molecules generated during complement activation induce a dramatic enhancement of particle uptake in rainbow trout phagocytes.|
|Autori:||LI, J; PETERS, R; LAPATRA, S; VAZZANA, M; SUNYER, JO|
|Tipologia:||Articolo su rivista|
|Citazione:||LI, J., PETERS, R., LAPATRA, S., VAZZANA, M., & SUNYER, J.O. (2004). Anaphylatoxin-like molecules generated during complement activation induce a dramatic enhancement of particle uptake in rainbow trout phagocytes. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 28, 1005-1021.|
|Digital Object Identifier (DOI):||10.1016/j.dci.2004.03.004|
|Appare nelle tipologie:||01 - Articolo su rivista|