An experimental investigation of the performance of a low-temperature refrigerating unit working with R22 and a comparison of its performance when operating with replacement HFC fluids in accordance with the European Regulation CE-1005/2009 are presented in this paper. Plant working efficiency was tested with R22, as baseline, and then compared with four different HFC fluids: R413A, R417A, R422A and R422D. The refrigerating unit was a vapour-compression plant equipped with a reciprocating double-cylinder compressor able to keep the cold room at -20ºC. Lower values of the temperature at the end of compression and polytrophic exponent can be achieved with the HFC tested. Substituting the R22 led to refrigerating plant to underperform. The COP was lower for all the replacement fluids showing inferior energy efficiency and higher energy consumption. The TEWI parameter was also evaluated and compared for all the fluids tested in the present investigation, suggesting TEWI increments substituting the original fluid.