Our investigation, performed by combining data from differential proteomic analyses carried-out using wild-type (Wt) and non-producing strains incubated in different growth conditions, showed that antibiotic production is always associated with the up-regulation of either specific enzymes of balhimycin (bal) biosynthetic gene cluster and enzymes related to central carbon metabolism, cell energy and redox balance. Thus this approach suggested new insights to improve fermentation technology strategies and revealed target genes for synthetic biology approaches aimed to improve antibiotic yield production.
Alduina, R., Renzone, R., Scaloni, A., Puglia, A.M., Gallo, G. (2012). From microbial proteomics to synthetic biology: Amycolatopsis balhimycina case. In CHEMICAL ENGINEERING TRANSACTIONS [10.3303/CET1227037].
From microbial proteomics to synthetic biology: Amycolatopsis balhimycina case
ALDUINA, Rosa;PUGLIA, Anna Maria;GALLO, Giuseppe
2012-01-01
Abstract
Our investigation, performed by combining data from differential proteomic analyses carried-out using wild-type (Wt) and non-producing strains incubated in different growth conditions, showed that antibiotic production is always associated with the up-regulation of either specific enzymes of balhimycin (bal) biosynthetic gene cluster and enzymes related to central carbon metabolism, cell energy and redox balance. Thus this approach suggested new insights to improve fermentation technology strategies and revealed target genes for synthetic biology approaches aimed to improve antibiotic yield production.File | Dimensione | Formato | |
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