In humans, IL-17 is a proinflammatory cytokine that plays a key role in the clearance of extracellular bacteria promoting cell infiltration and production of several cytokines and chemokines. Here, we report on three Ciona intestinalis IL-17 homologues (CiIL17-1, CiIL17-2, CiIL17-3). The gene organisation, phylogenetic tree and modelling supported the close relationship with the mammalian IL-17A and IL-17F suggesting that the C. intestinalis IL-17 genes share a common ancestor in the chordate lineages. Real time PCR analysis showed a prompt expression induced by LPS inoculation suggesting that they are involved in the first phase of inflammatory response. In situ hybridization assays disclosed that the genes transcription was upregulated in the pharynx, the main organ of the ascidian immune system, and expressed by hemocytes (granulocytes and univacuolar refractile granulocyte) inside the pharynx vessels.

Vizzini, A., Di Falco, F., Parrinello, D., Sanfratello, M.A., Mazzarella, C., Parrinello, N., et al. (2015). Ciona intestinalis interleukin 17-like genes expression is upregulated by LPS challenge. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 48(1), 129-137 [10.1016/j.dci.2014.09.014].

Ciona intestinalis interleukin 17-like genes expression is upregulated by LPS challenge

Vizzini, A;Di Falco, F;Parrinello, D;Sanfratello, MA;Mazzarella, C;Parrinello, N;Cammarata, M
2015

Abstract

In humans, IL-17 is a proinflammatory cytokine that plays a key role in the clearance of extracellular bacteria promoting cell infiltration and production of several cytokines and chemokines. Here, we report on three Ciona intestinalis IL-17 homologues (CiIL17-1, CiIL17-2, CiIL17-3). The gene organisation, phylogenetic tree and modelling supported the close relationship with the mammalian IL-17A and IL-17F suggesting that the C. intestinalis IL-17 genes share a common ancestor in the chordate lineages. Real time PCR analysis showed a prompt expression induced by LPS inoculation suggesting that they are involved in the first phase of inflammatory response. In situ hybridization assays disclosed that the genes transcription was upregulated in the pharynx, the main organ of the ascidian immune system, and expressed by hemocytes (granulocytes and univacuolar refractile granulocyte) inside the pharynx vessels.
Settore BIO/05 - Zoologia
Vizzini, A., Di Falco, F., Parrinello, D., Sanfratello, M.A., Mazzarella, C., Parrinello, N., et al. (2015). Ciona intestinalis interleukin 17-like genes expression is upregulated by LPS challenge. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 48(1), 129-137 [10.1016/j.dci.2014.09.014].
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10447/100356
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