The study was conducted on typical Sicilian cheeses made from raw cow milk (Caciocavallo Palermitano) and sheep milk (Vastedda della valle del Belice PDO e Pecorino PDO). Genotypic identification of the LAB isolates was carried out by 16S/23S rRNA sequencing. a total of 400 bacteria strains were isolated and genotypically identified as Lactobacillus spp., Lactoccoccus spp., Pediococcus spp., Leuconostoc spp., Enterococcus spp. and Streptococcus spp. Evaluation of bacteriocin-producing LAB was investigated in vitro using the “spot on the lawn” method (2) aganist Listeria monocytogenes, Staphylococcus aureus, Salmonella enteritidis and Escherichia coli. Results showed a predominance of LAB showing inhibition alone against Listeria monocytogenes.
Mancuso, I., Carrozzo, A., Ducato, B., Todaro, M., Miraglia, V., Macaluso, G., et al. (2013). BATTERI LATTICI CON ATTIVITA’ ANTIBATTERICA ISOLATI DA FORMAGGI TRADIZIONALI SICILIANI. In Atti XV Congresso Nazionale S.I.Di.L.V (pp.336-337).
BATTERI LATTICI CON ATTIVITA’ ANTIBATTERICA ISOLATI DA FORMAGGI TRADIZIONALI SICILIANI
TODARO, Massimo;
2013-01-01
Abstract
The study was conducted on typical Sicilian cheeses made from raw cow milk (Caciocavallo Palermitano) and sheep milk (Vastedda della valle del Belice PDO e Pecorino PDO). Genotypic identification of the LAB isolates was carried out by 16S/23S rRNA sequencing. a total of 400 bacteria strains were isolated and genotypically identified as Lactobacillus spp., Lactoccoccus spp., Pediococcus spp., Leuconostoc spp., Enterococcus spp. and Streptococcus spp. Evaluation of bacteriocin-producing LAB was investigated in vitro using the “spot on the lawn” method (2) aganist Listeria monocytogenes, Staphylococcus aureus, Salmonella enteritidis and Escherichia coli. Results showed a predominance of LAB showing inhibition alone against Listeria monocytogenes.
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