Gene expression microarrays allow a researcher to measure the simultaneous response of thousands of genes to external conditions. Affymetrix GeneChipr expression array technology has become a standard tool in medical research. Anyway, a preprocessing step is usually necessary in order to obtain a gene expression measure. Aim of this paper is to propose a calibration method to estimate the nominal concentration based on a non-linear mixed model. This method is an enhancement of a method proposed in Mineo et al. (2006). The relationship between raw intensities and concentration is obtained by using the Langmuir isotherm theory.

MINEO AM, AUGUGLIARO L, FEDE C, RUGGIERI M (2007). A Statistical Calibration Method based on Non-Linear Mixed Model for Affymetrix Probe Level Data. In Cssification and Data Analysis Group - Book of Short Papers (pp.97-100). MACERATA : EUM Edizioni Università di Macerata.

A Statistical Calibration Method based on Non-Linear Mixed Model for Affymetrix Probe Level Data

MINEO, Angelo;AUGUGLIARO, Luigi;RUGGIERI, Mariantonietta
2007-01-01

Abstract

Gene expression microarrays allow a researcher to measure the simultaneous response of thousands of genes to external conditions. Affymetrix GeneChipr expression array technology has become a standard tool in medical research. Anyway, a preprocessing step is usually necessary in order to obtain a gene expression measure. Aim of this paper is to propose a calibration method to estimate the nominal concentration based on a non-linear mixed model. This method is an enhancement of a method proposed in Mineo et al. (2006). The relationship between raw intensities and concentration is obtained by using the Langmuir isotherm theory.
CLADAG2007
Macerata
12-14 Settembre 2007
MINEO AM, AUGUGLIARO L, FEDE C, RUGGIERI M (2007). A Statistical Calibration Method based on Non-Linear Mixed Model for Affymetrix Probe Level Data. In Cssification and Data Analysis Group - Book of Short Papers (pp.97-100). MACERATA : EUM Edizioni Università di Macerata.
Proceedings (atti dei congressi)
MINEO AM; AUGUGLIARO L; FEDE C; RUGGIERI M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/8840
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