Gene expression microarrays allow a researcher to measure the simultaneous response of thousands of genes to external conditions. Affymetrix GeneChipr expression array technology has become a standard tool in medical research. Anyway, a preprocessing step is usually necessary in order to obtain a gene expression measure. Aim of this paper is to propose a calibration method to estimate the nominal concentration based on a non-linear mixed model. This method is an enhancement of a method proposed in Mineo et al. (2006). The relationship between raw intensities and concentration is obtained by using the Langmuir isotherm theory.
Mineo, A., Augugliaro, L., Fede, C., & Ruggieri, M. (2007). A Statistical Calibration Method based on Non-Linear Mixed Model for Affymetrix Probe Level Data. In Cssification and Data Analysis Group - Book of Short Papers (pp.97-100). MACERATA : EUM Edizioni Università di Macerata.
Autori: | Mineo, A.; Augugliaro, L.; Fede, C.; Ruggieri, M. |
Titolo: | A Statistical Calibration Method based on Non-Linear Mixed Model for Affymetrix Probe Level Data |
Nome del convegno: | CLADAG2007 |
Luogo del convegno: | Macerata |
Anno del convegno: | 12-14 Settembre 2007 |
Data di pubblicazione: | 2007 |
Citazione: | Mineo, A., Augugliaro, L., Fede, C., & Ruggieri, M. (2007). A Statistical Calibration Method based on Non-Linear Mixed Model for Affymetrix Probe Level Data. In Cssification and Data Analysis Group - Book of Short Papers (pp.97-100). MACERATA : EUM Edizioni Università di Macerata. |
Tipologia: | 0 - Proceedings (TIPOLOGIA NON ATTIVA) |
Appare nelle tipologie: | 0 - Proceedings (TIPOLOGIA NON ATTIVA) |