Acetaldehyde (ACD), ethanol first metabolite, is rewarding in rodents and humans; it induces “place preference”, is self-administer directly in the VTA, orally in an operant/conflict paradigm and increases DA neurons’ firing. This research aims at investigating DA2-receptor role in the reinstatement of acetaldehyde operant-drinking behaviour, following induction, maintenance and abstinence in the rat. Male Wistar rats are trained to orally self-administer ACD solution (3.2% v/v) or water, in an operant chamber under a FR1. Afterwards animals undergo cyclic periods of deprivation and relapse to ACD. The effect ofD2-receptor activation by quinpirole (0.03mg/kg,i.p.) on operant ACD self-administration is tested during relapse sessions. Rats show a peak-and-drop drinking pattern that reaches regular and higher values in the last training days. Quinpirole administration produces lever press reduction in ACD group when compared to basal intake(p<0.001) and to vehicle (p<0.05;p<0.001), while when treatment is suspended, rats reinstate lever presses for ACD. ACD incentive properties involve dopamine neurotransmission: D2-receptor activation is able to reduce reinstatement of operant drinking behaviour for ACD, following periods of abstinence, probably acting at a pre-synaptic level, thus reducing DA release in mesolimbic areas. These findings further support ACD pivotal role in ethanol central effects.

Brancato, A., Marino, R., Plescia, F., Sutera, F.M., Cannizzaro, C. (2013). Acetaldehyde operant self-administration in rats: focus on D2-receptor activation.. In Dopamine 2013.

Acetaldehyde operant self-administration in rats: focus on D2-receptor activation.

BRANCATO, Anna;MARINO, Rosa Anna Maria;PLESCIA, Fulvio;SUTERA, Flavia Maria;CANNIZZARO, Carla
2013-01-01

Abstract

Acetaldehyde (ACD), ethanol first metabolite, is rewarding in rodents and humans; it induces “place preference”, is self-administer directly in the VTA, orally in an operant/conflict paradigm and increases DA neurons’ firing. This research aims at investigating DA2-receptor role in the reinstatement of acetaldehyde operant-drinking behaviour, following induction, maintenance and abstinence in the rat. Male Wistar rats are trained to orally self-administer ACD solution (3.2% v/v) or water, in an operant chamber under a FR1. Afterwards animals undergo cyclic periods of deprivation and relapse to ACD. The effect ofD2-receptor activation by quinpirole (0.03mg/kg,i.p.) on operant ACD self-administration is tested during relapse sessions. Rats show a peak-and-drop drinking pattern that reaches regular and higher values in the last training days. Quinpirole administration produces lever press reduction in ACD group when compared to basal intake(p<0.001) and to vehicle (p<0.05;p<0.001), while when treatment is suspended, rats reinstate lever presses for ACD. ACD incentive properties involve dopamine neurotransmission: D2-receptor activation is able to reduce reinstatement of operant drinking behaviour for ACD, following periods of abstinence, probably acting at a pre-synaptic level, thus reducing DA release in mesolimbic areas. These findings further support ACD pivotal role in ethanol central effects.
27-mag-2013
Dopamine 2013
2013
515
http://www.corsiecongressi.com/dopamine/final_program.asp
Brancato, A., Marino, R., Plescia, F., Sutera, F.M., Cannizzaro, C. (2013). Acetaldehyde operant self-administration in rats: focus on D2-receptor activation.. In Dopamine 2013.
Proceedings (atti dei congressi)
Brancato, A; Marino, RAM; Plescia, F; Sutera, FM; Cannizzaro, C
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/75968
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