Gamma-aminobutyric acid (GABA) is the principal inhibitory neurotransmitter in the mammalian central nervous system and acts via ionotropic (GABAA-Rs) and metabotropic (GABAB-Rs) receptors. GABAA-Rs are Cl- selective hetero-pentameric channels assembled by combinations of 19 distinct gene products. Instead, GABAB-Rs are bi-subunit G-protein coupled receptors linked to K+ or Ca2+ channels. Dysfunctions of GABA-signaling (GS) cause psychotic disorders and correlate with epigenetic alterations, such as over-expression of DNA methyl transferase-1 which in turn imposes iper-methylation of GABA-regulated genes. The sea urchin embryo, which presents a rudimentary nervous system, offers a big opportunity to study the GS and its potential epigenetic implications in a simple eukaryote model. To this purpose, we performed a comprehensive in silico analysis of the sea urchin genome and identified a 450 Kb long cluster containing two genes encoding for the GABAB-R subunits, and two genes respectively encoding for a α/γ/ε-type and a β/ρ/δ/τ/π-type GABA B A-R subunit. From an evolutionary perspective, this result revealed a unique genomic organization of these genes in sea urchin. Next, to preliminarily evaluate the role of GS during development, Paracentrotus lividus embryos were cultured in the presence of GABA at concentrations ranging from 0.01 to 1.0 mM. Compared to controls, treated embryos showed aberrations in axial patterning, with a dose-dependent effect. In particular, at 48 hours post-fertilization control embryos were normal bilateral symmetric plutei whereas GABA-treated embryos displayed a radial organization with supranumerary spicules. Washout experiments allowed to determine that the period of sensitivity is restricted from the blastula to the gastrula stage. Altogether, these results suggest that dysregulation of GS affects the polarization of the ectoderm. Although preliminary, this study provide the first evidence of GS activity during development of echinoderms.

Casamirra, S., Spinelli, G., Cavalieri, V. (2012). Identification of GABA receptor genes and evidence of GABA signaling during embryogenesis of the sea urchin. In Frontiers in Molecular Biology - Epigenetics in Development and Disease (pp.44-44).

Identification of GABA receptor genes and evidence of GABA signaling during embryogenesis of the sea urchin

CASAMIRRA, Silvia;SPINELLI, Giovanni;CAVALIERI, Vincenzo
2012-01-01

Abstract

Gamma-aminobutyric acid (GABA) is the principal inhibitory neurotransmitter in the mammalian central nervous system and acts via ionotropic (GABAA-Rs) and metabotropic (GABAB-Rs) receptors. GABAA-Rs are Cl- selective hetero-pentameric channels assembled by combinations of 19 distinct gene products. Instead, GABAB-Rs are bi-subunit G-protein coupled receptors linked to K+ or Ca2+ channels. Dysfunctions of GABA-signaling (GS) cause psychotic disorders and correlate with epigenetic alterations, such as over-expression of DNA methyl transferase-1 which in turn imposes iper-methylation of GABA-regulated genes. The sea urchin embryo, which presents a rudimentary nervous system, offers a big opportunity to study the GS and its potential epigenetic implications in a simple eukaryote model. To this purpose, we performed a comprehensive in silico analysis of the sea urchin genome and identified a 450 Kb long cluster containing two genes encoding for the GABAB-R subunits, and two genes respectively encoding for a α/γ/ε-type and a β/ρ/δ/τ/π-type GABA B A-R subunit. From an evolutionary perspective, this result revealed a unique genomic organization of these genes in sea urchin. Next, to preliminarily evaluate the role of GS during development, Paracentrotus lividus embryos were cultured in the presence of GABA at concentrations ranging from 0.01 to 1.0 mM. Compared to controls, treated embryos showed aberrations in axial patterning, with a dose-dependent effect. In particular, at 48 hours post-fertilization control embryos were normal bilateral symmetric plutei whereas GABA-treated embryos displayed a radial organization with supranumerary spicules. Washout experiments allowed to determine that the period of sensitivity is restricted from the blastula to the gastrula stage. Altogether, these results suggest that dysregulation of GS affects the polarization of the ectoderm. Although preliminary, this study provide the first evidence of GS activity during development of echinoderms.
Settore BIO/11 - Biologia Molecolare
24-mag-2012
8th SIBBM Seminar - Frontiers in Molecular Biology - Epigenetics in Development and Disease
Palermo
2012
2012
2012
1
Online
http://sibbm2012.azuleon.org/
Casamirra, S., Spinelli, G., Cavalieri, V. (2012). Identification of GABA receptor genes and evidence of GABA signaling during embryogenesis of the sea urchin. In Frontiers in Molecular Biology - Epigenetics in Development and Disease (pp.44-44).
Proceedings (atti dei congressi)
Casamirra, S; Spinelli, G; Cavalieri, V
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/75425
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