Many microorganisms are able to degrade aliphatic hydrocarbons and a relationship between n-alkane utilization and storage compound synthesis has been described in bacteria. The Gram positive GC-rich n-alkane degrader Gordonia sp. strain SoCg, isolated from a long-term accidentally contaminated beach in Sicily, is able to grow on long n-alkanes up to. It carries a single copy of the alkane hydroxylase gene alkB on its chromosome and its alk cluster revealed a genomic organization similar to other alk clusters of alkane-degrading Gram positive bacteria. The alk gene expression, analysed by Real-time RT-PCR, is induced by n-hexadecane and n-triacontane and coupled to alkane consumption. Interestingly, SPME GC-MS analysis revealed extracellular production of hexadecyl-hexadecanoic acid (wax ester), during growth on n-triacontane. Degenerated oligonucleotides were used to PCR amplify the Wax Esther Synthase/Acyl-CoA:Diacylglycerol Acyltransferase encoding gene (atfa), responsible for intracellular wax esther synthesis by estherification between palmitoyl-CoA and 1-haxadecanol in the n-alkane degrader Acinetobacter sp. ADP1. The amplicon showed 75% similarity sequence to a putative acyl-CoA transferase gene of Nocardia farcinica. Only one copy of atfa-like gene was detected in Gordonia SoCg chromosome. qRT-PCR analysis showed an up-regulation of atfa gene in the presence of long chain n-alkanes, correlating, for the first time, long chain n-alkane metabolism and wax esthers extracellular production.

Lo Piccolo, L., De Pasquale, C., Tabone, M., Imparato, V., Fodale, R., Puglia, A.M., et al. (2009). Exploring long chain n-alkane metabolism in Gordonia sp. strain SoCg. In SIMGBM 28th NATIONAL MEETING (pp.164-164).

Exploring long chain n-alkane metabolism in Gordonia sp. strain SoCg

LO PICCOLO, Luca;DE PASQUALE, Claudio;FODALE, Roberta;PUGLIA, Anna Maria;QUATRINI, Paola
2009-01-01

Abstract

Many microorganisms are able to degrade aliphatic hydrocarbons and a relationship between n-alkane utilization and storage compound synthesis has been described in bacteria. The Gram positive GC-rich n-alkane degrader Gordonia sp. strain SoCg, isolated from a long-term accidentally contaminated beach in Sicily, is able to grow on long n-alkanes up to. It carries a single copy of the alkane hydroxylase gene alkB on its chromosome and its alk cluster revealed a genomic organization similar to other alk clusters of alkane-degrading Gram positive bacteria. The alk gene expression, analysed by Real-time RT-PCR, is induced by n-hexadecane and n-triacontane and coupled to alkane consumption. Interestingly, SPME GC-MS analysis revealed extracellular production of hexadecyl-hexadecanoic acid (wax ester), during growth on n-triacontane. Degenerated oligonucleotides were used to PCR amplify the Wax Esther Synthase/Acyl-CoA:Diacylglycerol Acyltransferase encoding gene (atfa), responsible for intracellular wax esther synthesis by estherification between palmitoyl-CoA and 1-haxadecanol in the n-alkane degrader Acinetobacter sp. ADP1. The amplicon showed 75% similarity sequence to a putative acyl-CoA transferase gene of Nocardia farcinica. Only one copy of atfa-like gene was detected in Gordonia SoCg chromosome. qRT-PCR analysis showed an up-regulation of atfa gene in the presence of long chain n-alkanes, correlating, for the first time, long chain n-alkane metabolism and wax esthers extracellular production.
2009
SIMGBM NATIONAL MEETING
Spoleto
11-13 giugno 2009
28th
1
Lo Piccolo, L., De Pasquale, C., Tabone, M., Imparato, V., Fodale, R., Puglia, A.M., et al. (2009). Exploring long chain n-alkane metabolism in Gordonia sp. strain SoCg. In SIMGBM 28th NATIONAL MEETING (pp.164-164).
Proceedings (atti dei congressi)
Lo Piccolo, L; De Pasquale, C; Tabone, M; Imparato, V; Fodale, R; Puglia, AM; Quatrini, P
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/57173
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