Point mutations in codon 12 and 13 of K-ras are frequently found in DNA of colorectal cancer. It has been suggested that particular mutations at these sites may be associated with specific tumour phenotypes. To shed light on the molecular mechanisms on which depends this specificity we set up a system of HT-29 cells stably transfected with a cDNA coding for K-rasG13D under the control of an inducible promoter. Proliferation assay performed on one of the positives clones, showed a decreased growth rate in response to K-rasG13D expression and preliminary gene expression analysis showed an up-regulation of the cell-cycle inhibitor p21 WAF1.

Bellavia, M., La Farina, M., Colomba, P., Eterno, V., Miranda, F., Albanese, I. (2010). Characterization of transfected HT-29 cells expressing the oncogenic Ras isoform KrasG13D.. In Excerpts from DBCS VII - Congresso Nazionale del Dipartimento di Biologia Cellulare e dello Sviluppo (pp.4-6). Università degli Studi di Palermo.

Characterization of transfected HT-29 cells expressing the oncogenic Ras isoform KrasG13D.

BELLAVIA, Maurizio;LA FARINA, Mario;COLOMBA, Paolo;ETERNO, Vincenzo;MIRANDA, Fabrizio;ALBANESE, Ida
2010-01-01

Abstract

Point mutations in codon 12 and 13 of K-ras are frequently found in DNA of colorectal cancer. It has been suggested that particular mutations at these sites may be associated with specific tumour phenotypes. To shed light on the molecular mechanisms on which depends this specificity we set up a system of HT-29 cells stably transfected with a cDNA coding for K-rasG13D under the control of an inducible promoter. Proliferation assay performed on one of the positives clones, showed a decreased growth rate in response to K-rasG13D expression and preliminary gene expression analysis showed an up-regulation of the cell-cycle inhibitor p21 WAF1.
Congresso Nazionale del Dipartimento di Biologia Cellulare e dello Sviluppo
2009
7
2010
3
http://www.unipa.it/dipbio/congresso2009/congresso2009.html
Bellavia, M., La Farina, M., Colomba, P., Eterno, V., Miranda, F., Albanese, I. (2010). Characterization of transfected HT-29 cells expressing the oncogenic Ras isoform KrasG13D.. In Excerpts from DBCS VII - Congresso Nazionale del Dipartimento di Biologia Cellulare e dello Sviluppo (pp.4-6). Università degli Studi di Palermo.
Proceedings (atti dei congressi)
Bellavia, M; La Farina, M; Colomba, P; Eterno, V; Miranda, F; Albanese, I
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/44554
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