Release of extracellular vesicles (EVs) is a process conserved from prokaryotes to eucaryotes. Although EVs are produced from both normal and cancer cells, malignant cells release a much higher amount of EVs, which contain tumour-specific proteins and RNAs. We previously found that G26/24 oligodendroglioma cells shed EVs that contain the pro-apoptotic factors FasL and TRAIL and are able to inhibit neurite outgrowth, and induce apoptosis in about 75% of rat cortical neurons [1] and 40% of astrocytes [2] in culture. By labelling proteins synthesized in one cell type, we also demonstrated EV-mediated horizontal transfer of proteins among brain cells. Interestingly, G2624 release, via EVs, extracellular matrix remodelling proteases [3], and H1° histone protein [4]. We suggested that by releasing H1°, a differentiation-specific histone, cancer cells may escape differentiation cues [4]. To shed further light on the role of EVs in discarding proteins and mRNAs otherwise able to counteract proliferative signals, we studied a melanoma cell line (A375). We found that also these cancer cells produce H1° and release it into EVs. Interestingly, H1° sorted to vesicles shows a molecular mass higher than expected, and is probably sumoylated. By T1 RNase-protection assay with the H1° RNA, three main complexes were evidenced in EVs, the most abundant of which has a molecular mass of about 65 kDa. By using a biotinylated H1°RNA to fish interacting factors, we isolated from EVs a few proteins which have been then identified by mass spectrometry: the most abundant is a protein of about 60 kDa, recognized as myelin expression factor-2 (MYEF2). Western blot analyses confirmed the presence of MYEF2 in EVs released from A375 melanoma cells. [1] D’Agostino et al- 2006. Int J Oncol 29:1075-85. [2] Lo Cicero A et al. 2011, Int J Oncol 39:1353-7 [3] Lo Cicero A et al. 2012, Matrix Biol 31:229-33 [4] Schiera G et al. 2013, Int J Oncol 43:1771-6

Schiera, G., Di Liegro, C., Puleo, V., Colletta, O., Di Liegro, I. (2015). Extracellular vesicles released from melanoma cells contain H1° mRNA-binding proteins, one of which is (probably) MYEF2.. In Proceedings of the Abstracts of the 20th World Congress on Advances in Oncology and 18th International Symposium on Molecular Medicine 08-10-October 2015; Athens , Greece. (pp.61-61). Athens : D. A. Spandidos.

Extracellular vesicles released from melanoma cells contain H1° mRNA-binding proteins, one of which is (probably) MYEF2.

Schiera G.;Di Liegro C. M.;Di Liegro I.
2015-01-01

Abstract

Release of extracellular vesicles (EVs) is a process conserved from prokaryotes to eucaryotes. Although EVs are produced from both normal and cancer cells, malignant cells release a much higher amount of EVs, which contain tumour-specific proteins and RNAs. We previously found that G26/24 oligodendroglioma cells shed EVs that contain the pro-apoptotic factors FasL and TRAIL and are able to inhibit neurite outgrowth, and induce apoptosis in about 75% of rat cortical neurons [1] and 40% of astrocytes [2] in culture. By labelling proteins synthesized in one cell type, we also demonstrated EV-mediated horizontal transfer of proteins among brain cells. Interestingly, G2624 release, via EVs, extracellular matrix remodelling proteases [3], and H1° histone protein [4]. We suggested that by releasing H1°, a differentiation-specific histone, cancer cells may escape differentiation cues [4]. To shed further light on the role of EVs in discarding proteins and mRNAs otherwise able to counteract proliferative signals, we studied a melanoma cell line (A375). We found that also these cancer cells produce H1° and release it into EVs. Interestingly, H1° sorted to vesicles shows a molecular mass higher than expected, and is probably sumoylated. By T1 RNase-protection assay with the H1° RNA, three main complexes were evidenced in EVs, the most abundant of which has a molecular mass of about 65 kDa. By using a biotinylated H1°RNA to fish interacting factors, we isolated from EVs a few proteins which have been then identified by mass spectrometry: the most abundant is a protein of about 60 kDa, recognized as myelin expression factor-2 (MYEF2). Western blot analyses confirmed the presence of MYEF2 in EVs released from A375 melanoma cells. [1] D’Agostino et al- 2006. Int J Oncol 29:1075-85. [2] Lo Cicero A et al. 2011, Int J Oncol 39:1353-7 [3] Lo Cicero A et al. 2012, Matrix Biol 31:229-33 [4] Schiera G et al. 2013, Int J Oncol 43:1771-6
The 20th World Congress on Advances in Oncology and 18th International Symposium on Molecular Medicine;
Athens , Greece.
dal 08-10-205 al 10-10-2015
20
2015
1
A stampa
abstract n 326, pagina S61
Schiera, G., Di Liegro, C., Puleo, V., Colletta, O., Di Liegro, I. (2015). Extracellular vesicles released from melanoma cells contain H1° mRNA-binding proteins, one of which is (probably) MYEF2.. In Proceedings of the Abstracts of the 20th World Congress on Advances in Oncology and 18th International Symposium on Molecular Medicine 08-10-October 2015; Athens , Greece. (pp.61-61). Athens : D. A. Spandidos.
Proceedings (atti dei congressi)
Schiera, G.; Di Liegro, C.; Puleo, V.; Colletta, O.; Di Liegro, I.
File in questo prodotto:
File Dimensione Formato  
Abstract Book 2015.pdf

Solo gestori archvio

Dimensione 3.73 MB
Formato Adobe PDF
3.73 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/248937
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? 0
social impact