Nanogels (NGs) have a great potential in the development of “smart” nanocarriers for (bio)molecular drugs and contrast agent for bioimaging. They are formed by physically or chemically crosslinked polymer networks, characterized by a large and flexible surface available for multivalent bioconjugations. NGs can be produced with high yields and through-puts by pulsed electron-beam irradiation of dilute aqueous solutions of water-soluble biocompatible polymers. In this work, a carboxyl functionalized nanogel system (NG), generated by pulsed e-beam irradiation of a semi-dilute poly(N-vinyl pyrrolidone) (PVP) aqueous solution in the presence of acrylic acid, with an average diameter in the 60-70 nm range (PDI<0.3) was used as a substrate to generate chemically stable insulin-grafted PVP NGs. In particular, grafting was carried out using human insulin without (PVP-g-insulin) or with fluorescein isothiocyanate labeling (PVP-g-insulin-FITC). The hydrodynamic dimensions of NGs before and after grafting (“naked NGs” and “grafted NGs”) were investigated by Dynamic Light Scattering. The PVP-g-insulin-FITC system was used in order to both quantify the conjugation degree of insulin to the nanoparticles by UV-vis spectroscopy and to study NGs localization in cell cultures. Different conjugation degrees were obtained by varying the reaction conditions. Biocompatibility tests of naked and insulin-grafted NGs were performed on neuroblastoma LAN5 cells by MTS assay. Colocalization of PVP-g-insulin-FITC NGs with activated insulin receptor was detected by immunohistochemistry technique and microscopical observations. Finally, the biological effect of insulin-grafted NGs was verified by activation of Akt and FOXO3a, two molecules involved in insulin signaling.

Picone, P., Ditta, L.A., Giacomazza, D., Sabatino, M.A., Nuzzo, D., Di Carlo, M., et al. (2015). Neuroprotective effect of a new Insulin-Nanogel system. In 59th annual meeting of Biophysical Society, Addendum & late Abstract listing.

Neuroprotective effect of a new Insulin-Nanogel system

Ditta, Lorena Anna;SABATINO, Maria Antonietta;DISPENZA, Clelia
2015-01-01

Abstract

Nanogels (NGs) have a great potential in the development of “smart” nanocarriers for (bio)molecular drugs and contrast agent for bioimaging. They are formed by physically or chemically crosslinked polymer networks, characterized by a large and flexible surface available for multivalent bioconjugations. NGs can be produced with high yields and through-puts by pulsed electron-beam irradiation of dilute aqueous solutions of water-soluble biocompatible polymers. In this work, a carboxyl functionalized nanogel system (NG), generated by pulsed e-beam irradiation of a semi-dilute poly(N-vinyl pyrrolidone) (PVP) aqueous solution in the presence of acrylic acid, with an average diameter in the 60-70 nm range (PDI<0.3) was used as a substrate to generate chemically stable insulin-grafted PVP NGs. In particular, grafting was carried out using human insulin without (PVP-g-insulin) or with fluorescein isothiocyanate labeling (PVP-g-insulin-FITC). The hydrodynamic dimensions of NGs before and after grafting (“naked NGs” and “grafted NGs”) were investigated by Dynamic Light Scattering. The PVP-g-insulin-FITC system was used in order to both quantify the conjugation degree of insulin to the nanoparticles by UV-vis spectroscopy and to study NGs localization in cell cultures. Different conjugation degrees were obtained by varying the reaction conditions. Biocompatibility tests of naked and insulin-grafted NGs were performed on neuroblastoma LAN5 cells by MTS assay. Colocalization of PVP-g-insulin-FITC NGs with activated insulin receptor was detected by immunohistochemistry technique and microscopical observations. Finally, the biological effect of insulin-grafted NGs was verified by activation of Akt and FOXO3a, two molecules involved in insulin signaling.
Settore CHIM/07 - Fondamenti Chimici Delle Tecnologie
feb-2015
59th annual meeting of Biophysical Society
Baltimore, Maryland
7-11/02/2015
2015
1
Picone, P., Ditta, L.A., Giacomazza, D., Sabatino, M.A., Nuzzo, D., Di Carlo, M., et al. (2015). Neuroprotective effect of a new Insulin-Nanogel system. In 59th annual meeting of Biophysical Society, Addendum & late Abstract listing.
Proceedings (atti dei congressi)
Picone, P; Ditta, L A; Giacomazza, D; Sabatino, M A; Nuzzo, D; Di Carlo, M; San Biagio, P L; Dispenza, C
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/166326
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