The use of cell cultures involves different fields of biology, from diagnosis to research. Moreover, technologies based on animal cells represent a useful tool to the development of biological products for the prophylaxis and therapy in humans and animals. Therefore, it is necessary to perform quality controls, including virological tests. Several tests performed in research laboratories are able to discriminate one or more viral species, but it is not possible to demonstrate the presence of contaminant viral genome with one non-specific method. The aim of this work consisted on the realization of a biomolecular method able to detect and to identify by sequencing extraneous viral genome in cell cultures of animal and human origin in the absence of any specific information about the virus.
Di Bella, S., Di Marco, P., Russotto, L., Cannella, V., Altomare, R., Purpari, G., et al. (2012). An innovative method for the detection of contaminant viral genome in cell cultures. In Atti del 25° Convegno Annuale della Associazione Italiana di Colture Cellulari (ONLUS – AICC). Controllo dei processi di proliferazione e morte cellulare. Nuovi svciluppi della ricerca oncologica preclinica. International Satellite Symposium AICC – GISM. Mesenchymal stem cells: growth factors and cytokines.
An innovative method for the detection of contaminant viral genome in cell cultures
DI BELLA, Santina;ALTOMARE, Roberta;PURPARI, Giuseppa;
2012-01-01
Abstract
The use of cell cultures involves different fields of biology, from diagnosis to research. Moreover, technologies based on animal cells represent a useful tool to the development of biological products for the prophylaxis and therapy in humans and animals. Therefore, it is necessary to perform quality controls, including virological tests. Several tests performed in research laboratories are able to discriminate one or more viral species, but it is not possible to demonstrate the presence of contaminant viral genome with one non-specific method. The aim of this work consisted on the realization of a biomolecular method able to detect and to identify by sequencing extraneous viral genome in cell cultures of animal and human origin in the absence of any specific information about the virus.
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