Data on evaluation of some diagnostic parameters of all ELISA kits available in Italy for the serodiagnosis of AIE are presented and discussed. Ten laboratories were involved using a panel of 30 sera ran with 4 commercial kits and 2 in-house kits. Kits were also evaluated using a panel of sera from vaccinated animals at different days post vaccination (p.v.). All sera were also tested in agar gel immunodiffusion (AGID). The parameters evaluated were: diagnostic sensitivity (DSe) and specificity (DSp), Cohen K, weighted Cohen K, coefficient of variation (CV), accordance, concordance. Analysis of these parameters indicates that all kits have a higher sensitivity than AGID, even if a complete evaluation, as indicated by OIE (1) should be carried out.

Nardini, R., Scicluna, M., Terregino, C., Mandola, M., Cavaliere, N., Cordioli, P., et al. (2012). Valutazione di alcune performance diagnostiche di kit ELISA per la diagnosi sierologica di Anemia Infettiva Equina (AIE). In Atti del XIV Congresso Nazionale S.I.Di.L.V..

Valutazione di alcune performance diagnostiche di kit ELISA per la diagnosi sierologica di Anemia Infettiva Equina (AIE)

PURPARI, Giuseppa;
2012-01-01

Abstract

Data on evaluation of some diagnostic parameters of all ELISA kits available in Italy for the serodiagnosis of AIE are presented and discussed. Ten laboratories were involved using a panel of 30 sera ran with 4 commercial kits and 2 in-house kits. Kits were also evaluated using a panel of sera from vaccinated animals at different days post vaccination (p.v.). All sera were also tested in agar gel immunodiffusion (AGID). The parameters evaluated were: diagnostic sensitivity (DSe) and specificity (DSp), Cohen K, weighted Cohen K, coefficient of variation (CV), accordance, concordance. Analysis of these parameters indicates that all kits have a higher sensitivity than AGID, even if a complete evaluation, as indicated by OIE (1) should be carried out.
ott-2012
XIV Congresso Nazionale S.I.Di.L.V.
Sorrento - Napoli
24/26 ottobre 2012
4
Nardini, R., Scicluna, M., Terregino, C., Mandola, M., Cavaliere, N., Cordioli, P., et al. (2012). Valutazione di alcune performance diagnostiche di kit ELISA per la diagnosi sierologica di Anemia Infettiva Equina (AIE). In Atti del XIV Congresso Nazionale S.I.Di.L.V..
Proceedings (atti dei congressi)
Nardini, R.; Scicluna, M.; Terregino, C.; Mandola, M.; Cavaliere, N.; Cordioli, P.; Fusco, G.; Purpari, G.; Angioini, A.; Panzieri, C.; Manca, M.; Autorino, G.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/118389
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