The mRNA and protein levels of RKIP are reduced and those of YY1 increased in clinical HCC. Loss, mutation, or promoter hypermethylation of the RKIP gene may not account for the downregulation of RKIP in HCC. Histone deacetylation can silence gene expression and play a significant role in hepatocarcinogenesis. The histone deacetylase inhibitor (HDACI) trichostatin induced cell growth inhibition and proapoptotic effects in HA22T/VGH and HepG2 HCC cells; it also exhibited synergy with doxorubicin. Treatment with trichostatin caused histone hyperacetylation and down- or upregulated expression of different genes (such as β-catenin, cyclin D1, hTERT, XIAP, and IL-6). These changes might, at least in part, explain the anticancer and chemosensitizing activities of trichostatin. Nevertheless, trichostatin did not modify RKIP or YY1 mRNA and protein levels in the two representative HCC cell lines. Although further studies are necessary to clarify the possible role of epigenetic changes in the expression of RKIP and YY1, our results underline the therapeutic potential of HDACIs in HCC

Inguglia, L., Notarbartolo di Villarosa, M., Poma, P., Labbozzetta, M., D'Alessandro, N. (2011). Induction of Apoptosis and Chemosensitization by the Histone Deacetylase Inhibitor Trichostatin in Hepatocellular Carcinoma Cells: Molecular Analysis and RKIP Levels. FORUM ON IMMUNOPATHOLOGICAL DISEASES AND THERAPEUTICS, 2(2), 127-135 [10.1615/ForumImmunDisTher.v2.i2.40].

Induction of Apoptosis and Chemosensitization by the Histone Deacetylase Inhibitor Trichostatin in Hepatocellular Carcinoma Cells: Molecular Analysis and RKIP Levels

INGUGLIA, Luigi;NOTARBARTOLO DI VILLAROSA, Monica;POMA, Paola;LABBOZZETTA, Manuela;D'ALESSANDRO, Natale
2011-01-01

Abstract

The mRNA and protein levels of RKIP are reduced and those of YY1 increased in clinical HCC. Loss, mutation, or promoter hypermethylation of the RKIP gene may not account for the downregulation of RKIP in HCC. Histone deacetylation can silence gene expression and play a significant role in hepatocarcinogenesis. The histone deacetylase inhibitor (HDACI) trichostatin induced cell growth inhibition and proapoptotic effects in HA22T/VGH and HepG2 HCC cells; it also exhibited synergy with doxorubicin. Treatment with trichostatin caused histone hyperacetylation and down- or upregulated expression of different genes (such as β-catenin, cyclin D1, hTERT, XIAP, and IL-6). These changes might, at least in part, explain the anticancer and chemosensitizing activities of trichostatin. Nevertheless, trichostatin did not modify RKIP or YY1 mRNA and protein levels in the two representative HCC cell lines. Although further studies are necessary to clarify the possible role of epigenetic changes in the expression of RKIP and YY1, our results underline the therapeutic potential of HDACIs in HCC
2011
Settore BIO/14 - Farmacologia
Inguglia, L., Notarbartolo di Villarosa, M., Poma, P., Labbozzetta, M., D'Alessandro, N. (2011). Induction of Apoptosis and Chemosensitization by the Histone Deacetylase Inhibitor Trichostatin in Hepatocellular Carcinoma Cells: Molecular Analysis and RKIP Levels. FORUM ON IMMUNOPATHOLOGICAL DISEASES AND THERAPEUTICS, 2(2), 127-135 [10.1615/ForumImmunDisTher.v2.i2.40].
File in questo prodotto:
File Dimensione Formato  
forum 2011_light.pdf

Solo gestori archvio

Descrizione: Articolo principale ottenuto tramite scansione
Tipologia: Versione Editoriale
Dimensione 5.99 MB
Formato Adobe PDF
5.99 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/53429
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 2
  • ???jsp.display-item.citation.isi??? ND
social impact