A bacterial artificial chromosomal library of Nonomuraea sp. ATCC39727 was constructed using Escherichia coli-Streptomyces artificial chromosome (ESAC) and screened for the presence of dbv genes known to be involved in the biosynthesis of the glycopeptide A40926. dbv genes were cloned as two large, partially overlapping, fragments and transferred into the host Streptomyces lividans, thus generating strains S. lividansColon, two colonsNmESAC50 and S. lividansColon, two colonsNmESAC57. The heterologous expression of Nonomuraea genes in S. lividans was successfully demonstrated by using combined RT-PCR and proteomic approaches. MALDI-TOF analysis revealed that a Nonomuraea ABC transporter is expressed as two isoforms in S. lividans. Moreover, its expression may not require a Nonomuraea positive regulator at all, as it is present at similar levels in both clones even though S. lividansColon, two colonsNmESAC57 lacks regulatory genes. Considered together, these results show that S. lividans expresses Nonomuraea genes from their own promoters and support the idea that S. lividans can be a good host for genetic analysis of Nonomuraea.

Alduina R, Giardina A, Gallo G, Renzone G, Ferraro C, Contino A, et al. (2005). Expression in Streptomyces lividans of Nonomuraea genes cloned in an artificial chromosome. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 68, 656-662 [10.1007/s00253-005-1929-y].

Expression in Streptomyces lividans of Nonomuraea genes cloned in an artificial chromosome

ALDUINA, Rosa;GIARDINA, Anna;GALLO, Giuseppe;FERRARO, Clelia;CONTINO, Alba;PUGLIA, Anna Maria
2005-01-01

Abstract

A bacterial artificial chromosomal library of Nonomuraea sp. ATCC39727 was constructed using Escherichia coli-Streptomyces artificial chromosome (ESAC) and screened for the presence of dbv genes known to be involved in the biosynthesis of the glycopeptide A40926. dbv genes were cloned as two large, partially overlapping, fragments and transferred into the host Streptomyces lividans, thus generating strains S. lividansColon, two colonsNmESAC50 and S. lividansColon, two colonsNmESAC57. The heterologous expression of Nonomuraea genes in S. lividans was successfully demonstrated by using combined RT-PCR and proteomic approaches. MALDI-TOF analysis revealed that a Nonomuraea ABC transporter is expressed as two isoforms in S. lividans. Moreover, its expression may not require a Nonomuraea positive regulator at all, as it is present at similar levels in both clones even though S. lividansColon, two colonsNmESAC57 lacks regulatory genes. Considered together, these results show that S. lividans expresses Nonomuraea genes from their own promoters and support the idea that S. lividans can be a good host for genetic analysis of Nonomuraea.
2005
Alduina R, Giardina A, Gallo G, Renzone G, Ferraro C, Contino A, et al. (2005). Expression in Streptomyces lividans of Nonomuraea genes cloned in an artificial chromosome. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 68, 656-662 [10.1007/s00253-005-1929-y].
File in questo prodotto:
File Dimensione Formato  
Expression in Streptomyces lividans_2005.pdf

Solo gestori archvio

Descrizione: Articolo principale
Dimensione 250.55 kB
Formato Adobe PDF
250.55 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/5290
Citazioni
  • ???jsp.display-item.citation.pmc??? 6
  • Scopus 17
  • ???jsp.display-item.citation.isi??? 14
social impact