Extra-intestinalE.coliareemergingasaglobalthreatduetotheirdiffusionasopportunisticpathogensand,aboveall,totheirwide setofantibioticresistancedeterminants.Therearestillmanygapsinourknowledgeoftheiroriginandspreadpathways,although food animalshavebeenadjudicatedvehiclesfor passingmult-drugresistantbacteriatohumans.Thisstudyanalyzed 46samples of meat purchasedfrom retail stores in Palermo in order toobtain quinolone-resistant E. coli isolates. Strains were screenedfor their phylogeneticgroups, ST131-associatedsingle nucleotidepolymorphisms (SNPs),andthentypedbyERIC-PCR. Theirsetof virulencefactors,namely,kpsMII,papA,sfaS,focG,iutA,papC,hlyD,andafagenes,wereinvestigatedandtheirfluoroquinoloneresistancedeterminantsevaluated.Thedataobtainedshowadramaticallyhighprevalenceofmultidrugresistancepatternsinthe Palermoarea,with28%oftheisolateshavingvirulencefactorgenestypicalofExPEC strains.NoB2grouporST131 strainswere detected.Moreover,20%ofourisolatesshowedpositivitytoalltheplasmid-mediatedquinoloneresistance(PMQR)determinants, showingapotentialtotransferthesegenesamongotherbacteria.Therefore,thesedataunderlinethepossibilitythatfoodanimals and,specifically,poultryinparticularmaybeasignificantsourceofresistantbacterialstrains,posingapotentialzoonoticrisk.
Giorgia Caruso, A.G. (2018). Extra-Intestinal Fluoroquinolone-Resistant Escherichia coli Strains Isolated from Meat. BIOMED RESEARCH INTERNATIONAL, 1-8 [10.1155/2018/8714975].
Extra-Intestinal Fluoroquinolone-Resistant Escherichia coli Strains Isolated from Meat
Anna Giammanco;Giuseppina Capra;Teresa Fasciana.
2018-01-01
Abstract
Extra-intestinalE.coliareemergingasaglobalthreatduetotheirdiffusionasopportunisticpathogensand,aboveall,totheirwide setofantibioticresistancedeterminants.Therearestillmanygapsinourknowledgeoftheiroriginandspreadpathways,although food animalshavebeenadjudicatedvehiclesfor passingmult-drugresistantbacteriatohumans.Thisstudyanalyzed 46samples of meat purchasedfrom retail stores in Palermo in order toobtain quinolone-resistant E. coli isolates. Strains were screenedfor their phylogeneticgroups, ST131-associatedsingle nucleotidepolymorphisms (SNPs),andthentypedbyERIC-PCR. Theirsetof virulencefactors,namely,kpsMII,papA,sfaS,focG,iutA,papC,hlyD,andafagenes,wereinvestigatedandtheirfluoroquinoloneresistancedeterminantsevaluated.Thedataobtainedshowadramaticallyhighprevalenceofmultidrugresistancepatternsinthe Palermoarea,with28%oftheisolateshavingvirulencefactorgenestypicalofExPEC strains.NoB2grouporST131 strainswere detected.Moreover,20%ofourisolatesshowedpositivitytoalltheplasmid-mediatedquinoloneresistance(PMQR)determinants, showingapotentialtotransferthesegenesamongotherbacteria.Therefore,thesedataunderlinethepossibilitythatfoodanimals and,specifically,poultryinparticularmaybeasignificantsourceofresistantbacterialstrains,posingapotentialzoonoticrisk.
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