Tooth replantation, as a treatment concept, has been subject to controversies regarding the mechanism as well as the various parameters underlying this process. This work aimed to study time-related changes in the pulp of replanted mature human premolars through the changes in the levels of certain factors involved in the underlying mechanisms of pulpal tissue healing after replantation. Eleven experimental mature teeth were extracted, immediately replanted in the original socket and left without any other intervention for 1, 2, 3 and 12 weeks before re-extraction. Three premolars served as control. All specimens were subject to histological analysis and the levels of MMP-2, MMP-9, Annexin V, iNOS and BCL-2 (anti-apoptotic family) were analyzed employing immunohistochemistry. The results showed degradation of the extracellular matrix (ECM), inflammatory cell infiltrate, loss in pulpo-dentine interface and loss of odontoblasts in the dental pulp tissue. This was accompanied by increase over time of MMP-9, Annexin V, iNOS and a decrease of BCL-2 and MMP-2, suggesting that apoptosis increased throughout the experimental period.

Leone, A., Angelova Volponi, A., Uzzo, M.L., Spatola, G.F., Jurjus, A., Vandevska-Radunovic, V. (2015). Dental pulp in mature human teeth: morphological alterations and metalloproteinases-2 and -9, Annexin-5, BCL-2, and iNOS modulation. JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS, 4(29), 143-149.

Dental pulp in mature human teeth: morphological alterations and metalloproteinases-2 and -9, Annexin-5, BCL-2, and iNOS modulation.

LEONE, Angelo;UZZO, Maria Laura;SPATOLA, Giovanni Francesco;
2015-01-01

Abstract

Tooth replantation, as a treatment concept, has been subject to controversies regarding the mechanism as well as the various parameters underlying this process. This work aimed to study time-related changes in the pulp of replanted mature human premolars through the changes in the levels of certain factors involved in the underlying mechanisms of pulpal tissue healing after replantation. Eleven experimental mature teeth were extracted, immediately replanted in the original socket and left without any other intervention for 1, 2, 3 and 12 weeks before re-extraction. Three premolars served as control. All specimens were subject to histological analysis and the levels of MMP-2, MMP-9, Annexin V, iNOS and BCL-2 (anti-apoptotic family) were analyzed employing immunohistochemistry. The results showed degradation of the extracellular matrix (ECM), inflammatory cell infiltrate, loss in pulpo-dentine interface and loss of odontoblasts in the dental pulp tissue. This was accompanied by increase over time of MMP-9, Annexin V, iNOS and a decrease of BCL-2 and MMP-2, suggesting that apoptosis increased throughout the experimental period.
2015
Settore BIO/17 - Istologia
Leone, A., Angelova Volponi, A., Uzzo, M.L., Spatola, G.F., Jurjus, A., Vandevska-Radunovic, V. (2015). Dental pulp in mature human teeth: morphological alterations and metalloproteinases-2 and -9, Annexin-5, BCL-2, and iNOS modulation. JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS, 4(29), 143-149.
File in questo prodotto:
File Dimensione Formato  
REPLANTED TEETH .pdf

Solo gestori archvio

Dimensione 431.29 kB
Formato Adobe PDF
431.29 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/201569
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 2
  • ???jsp.display-item.citation.isi??? ND
social impact