We have developed a simple and inexpensive device to obtain linear sucrose gradients with commonly used laboratory materials--a syringe, a flask, a plastic tube, and a piece of Pongo (Play-Doh). Refractive index values measured on sucrose fractions collected using our system demonstrate both the linearity and reliability of the gradients obtained.
BELLAVIA D, CELLURA D, SISINO G, BARBIERI R (2008). A homemade device for linear sucrose gradients. ANALYTICAL BIOCHEMISTRY, ?, ?-?.
A homemade device for linear sucrose gradients
BELLAVIA, Daniele;CELLURA, Dario;BARBIERI, Rainero
2008-01-01
Abstract
We have developed a simple and inexpensive device to obtain linear sucrose gradients with commonly used laboratory materials--a syringe, a flask, a plastic tube, and a piece of Pongo (Play-Doh). Refractive index values measured on sucrose fractions collected using our system demonstrate both the linearity and reliability of the gradients obtained.
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