Production of nitrogen dioxide by the activity of myeloperoxidase (MPO) in the presence of nitrite is now considered a key step in the pathophysiology of low-density lipoprotein (LDL) oxidation. This study shows that betanin, a phytochemical of the betalain class, inhibits the production of lipid hydroperoxides in human LDL submitted to a MPO/nitrite-induced oxidation. Kinetic measurements including time-course of particle oxidation and betanin consumption, either in the presence or in the absence of nitrite, suggest that the antioxidant effect is possibly the result of various actions. Betanin scavenges the initiator radical nitrogen dioxide and can also act as a lipoperoxyl radical-scavenger. In addition, unidentified oxidation product(s) of betanin by MPO/nitrite inhibit(s) the MPO/nitrite-induced LDL oxidation as effectively as the parent compound. In the light of betanin bioavailability and post-absorbtion distribution in humans, present findings may suggest favourable in vivo activity of this phytochemical.

ALLEGRA, M., FURTMULLER, P.G., JANTSCHKO, W., ZEDERBAUER, M., TESORIERE, L., LIVREA, M.A., et al. (2005). Mechanism of interaction of betanin and indicaxanthin with human myeloperoxidase and hypochlorous acid. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 332, 837-844 [10.1016/j.bbrc.2005.05.031].

Mechanism of interaction of betanin and indicaxanthin with human myeloperoxidase and hypochlorous acid

ALLEGRA, Mario;TESORIERE, Luisa;LIVREA, Maria Antonia;
2005-01-01

Abstract

Production of nitrogen dioxide by the activity of myeloperoxidase (MPO) in the presence of nitrite is now considered a key step in the pathophysiology of low-density lipoprotein (LDL) oxidation. This study shows that betanin, a phytochemical of the betalain class, inhibits the production of lipid hydroperoxides in human LDL submitted to a MPO/nitrite-induced oxidation. Kinetic measurements including time-course of particle oxidation and betanin consumption, either in the presence or in the absence of nitrite, suggest that the antioxidant effect is possibly the result of various actions. Betanin scavenges the initiator radical nitrogen dioxide and can also act as a lipoperoxyl radical-scavenger. In addition, unidentified oxidation product(s) of betanin by MPO/nitrite inhibit(s) the MPO/nitrite-induced LDL oxidation as effectively as the parent compound. In the light of betanin bioavailability and post-absorbtion distribution in humans, present findings may suggest favourable in vivo activity of this phytochemical.
2005
ALLEGRA, M., FURTMULLER, P.G., JANTSCHKO, W., ZEDERBAUER, M., TESORIERE, L., LIVREA, M.A., et al. (2005). Mechanism of interaction of betanin and indicaxanthin with human myeloperoxidase and hypochlorous acid. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 332, 837-844 [10.1016/j.bbrc.2005.05.031].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/1304
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